Recombinant humaan HSP70B/HSPA6-eiwit

HSP70B is a unique member of the human Hsp70 chaperone involved in cellular processes such as protein trafficking, folding, and prevention of aggregation. This protein is strictly stress-inducible, having little or no basal expression levels in most cells. HSP70B and Hsp72 are closely related and play cooperative roles in cell survival of proteotoxic stress. Recombinant human HSP70B, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Heat shock 70 kDa protein 6, HSPA6', Heat shock 70 kDa protein 6 Heat shock 70 kDa protein B'

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

73.2 kDa (663aa)

HSP70B, Heat shock 70 kDa protein 6, HSPA6', Heat shock 70 kDa protein 6 Heat shock 70 kDa protein B', ATGP0428-10 µg, ATGP0428-20 µg, ATGP0428-50 µg, ATGP0428-100 µg, ATGP0428-250 µg, ATGP0428-500 µg, ATGP0428-1 mg, ATGP0428-10, ATGP0428-20, ATGP0428-50, ATGP0428-100, ATGP0428-250, ATGP0428-500, ATGP0428-1

Noonan E., et al. (2008) . Exp Cell Res. 314 (13) :2468-76.; ; Hightower LE., et al. (2007) . Cell Stress Chaperones. 12 (4) :393-402.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 10% glycerol

Heat Shock Proteins

NP_002146

P17066

Human

MQAPRELAVG IDLGTTYSCV GVFQQGRVEI LANDQGNRTT PSYVAFTDTE RLVGDAAKSQ AALNPHNTVF DAKRLIGRKF ADTTVQSDMK HWPFRVVSEG GKPKVRVCYR GEDKTFYPEE ISSMVLSKMK ETAEAYLGQP VKHAVITVPA YFNDSQRQAT KDAGAIAGLN VLRIINEPTA AAIAYGLDRR GAGERNVLIF DLGGGTFDVS VLSIDAGVFE VKATAGDTHL GGEDFDNRLV NHFMEEFRRK HGKDLSGNKR ALRRLRTACE RAKRTLSSST QATLEIDSLF EGVDFYTSIT RARFEELCSD LFRSTLEPVE KALRDAKLDK AQIHDVVLVG GSTRIPKVQK LLQDFFNGKE LNKSINPDEA VAYGAAVQAA VLMGDKCEKV QDLLLLDVAP LSLGLETAGG VMTTLIQRNA TIPTKQTQTF TTYSDNQPGV FIQVYEGERA MTKDNNLLGR FELSGIPPAP RGVPQIEVTF DIDANGILSV TATDRSTGKA NKITITNDKG RLSKEEVERM VHEAEQYKAE DEAQRDRVAA KNSLEAHVFH VKGSLQEESL RDKIPEEDRR KMQDKCREVL AWLEHNQLAE KEEYEHQKRE LEQICRPIFS RLYGGPGVPG GSSCGTQARQ GDPSTGPIIE EVD