Recombinant humaan HSPB7-eiwit

HSPB7 is a member of HSPB (Heat shock protein beta) family. The HSPB family is one of the more diverse families within the group of HSP families. Some members have chaperone-like activities and/or play a role in cytoskeletal stabilization. Some members also show a dynamic, stress-induced translocation to SC35 splicing speckles. HSPB7 constitutively localized to SC35 splicing speckles, driven by its N-terminus. unlike HSPB1 and HSPB5, that chaperoned heat unfolded substrates and kept them folding competent, HSPB7 did not support refolding. Recombinant HSPB7 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Heat shock protein beta-7, cvHSP, DKFZp779D0968, FLJ32733, Heat shock protein beta-7 cvhsp, Heat shock 27kda protein family member 7, Heat shock protein beta 7, hspb7, Hypothetical protein flj34956.

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

20.7 kDa (190aa) confirmed by MALDI-TOF

HSPB7, Heat shock protein beta-7, cvHSP, DKFZp779D0968, FLJ32733, Heat shock protein beta-7 cvhsp, Heat shock 27kda protein family member 7, Heat shock protein beta 7, hspb7, Hypothetical protein flj34956., ATGP0511-10 µg, ATGP0511-20 µg, ATGP0511-50 µg, ATGP0511-100 µg, ATGP0511-250 µg, ATGP0511-500 µg, ATGP0511-1 mg, ATGP0511-10, ATGP0511-20, ATGP0511-50, ATGP0511-100, ATGP0511-250, ATGP0511-500, ATGP0511-1

VOS MJ., et al. (2009) Biochim Biophys Acta. 1793 (8) :1343-53.; ; Matkovich SJ, et al. (2010) J Clin Invest. 120 (1) :280-9;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 200 mM NaCl, 2mM DTT, 50% glycerol

Heat Shock Proteins

NP_055239

Q9UBY9

Human

MGSSHHHHHH SSGLVPRGSH MSHRTSSTFR AERSFHSSSS SSSSSTSSSA SRALPAQDPP MEKALSMFSD DFGSFMRPHS EPLAFPARPG GAGNIKTLGD AYEFAVDVRD FSPEDIIVTT SNNHIEVRAE KLAADGTVMN TFAHKCQLPE DVDPTSVTSA LREDGSLTIR ARRHPHTEHV QQTFRTEIKI