Recombinant humaan HSPB8-eiwit

Heat shock protein beta-8 (HSPB8), also known as HSP22, member of the small heat shock protein superfamily. HSPB8 is predominantly transcribed in skeletal muscle and heart. The chaperone-like activity is of great importance to the function of HSPB8 in various processes including proliferation, apoptosis and macroautophagy. Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) . Recombinant human HSPB8, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Heat shock protein beta-8, CRYAC, E2IG1, CMT2L, DHMN2, HMN2, HMN2A, Heat shock protein beta-8, heat shock 22kDa protein 8, H11, HSP22, HspB8

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.7 kDa (216aa) confirmed by MALDI-TOF

HSPB8, Heat shock protein beta-8, CRYAC, E2IG1, CMT2L, DHMN2, HMN2, HMN2A, Heat shock protein beta-8, heat shock 22kDa protein 8, H11, HSP22, HspB8, ATGP0493-10 µg, ATGP0493-20 µg, ATGP0493-50 µg, ATGP0493-100 µg, ATGP0493-250 µg, ATGP0493-500 µg, ATGP0493-1 mg, ATGP0493-10, ATGP0493-20, ATGP0493-50, ATGP0493-100, ATGP0493-250, ATGP0493-500, ATGP0493-1

Sun X., et al. (2004) J Biol Chem. 279 (4) :2394-402. ; ; Benndorf R., et al. (2001) J Biol Chem. 276:26753-61. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 20% glycerol

Heat Shock Proteins

NP_055180.1

Q9UJY1

Human

MADGQMPFSC HYPSRLRRDP FRDSPLSSRL LDDGFGMDPF PDDLTASWPD WALPRLSSAW PGTLRSGMVP RGPTATARFG VPAEGRTPPP FPGEPWKVCV NVHSFKPEEL MVKTKDGYVE VSGKHEEKQQ EGGIVSKNFT KKIQLPAEVD PVTVFASLSP EGLLIIEAPQ VPPYSTFGES SFNNELPQDS QEVTCT