Recombinant humaan HUS1-eiwit

HuS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex. This protein associates with Rad9 and Rad1 to form the 9-1-1 (RAD9-RAD1-HuS1) complex, which localizes to DNA lesions and promotes DNA damage signaling and repair or apoptosis, cell cycle arrest. The trimeric complex is structurally similar to the proliferating cell nuclear antigen (PCNA) sliding clamp and interacts with Rad17 as a clamp-clamp loader pair during the DNA damage response. Recombinant human HuS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Checkpoint protein HuS1, HUS1 checkpoint clamp component, HUS1 checkpoint homolog, hus1+-like protein, hHUS1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

33.8 kDa (300aa) confirmed by MALDI-TOF

HuS1, checkpoint protein HuS1, ATGP0815-10 µg, ATGP0815-20 µg, ATGP0815-50 µg, ATGP0815-100 µg, ATGP0815-250 µg, ATGP0815-500 µg, ATGP0815-1 mg, ATGP0815-10, ATGP0815-20, ATGP0815-50, ATGP0815-100, ATGP0815-250, ATGP0815-500, ATGP0815-1

Weiss RS., et al (2009) Proc Natl Acad Sci u S A. 106 (50) :21282-7.; ; Roos-Mattjus P., et al (2002) J Biol Chem. 15; ;277 (46) :43809-12.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 40% glycerol

Cell Cycle

NP_004498

O60921

Human

MKFRAKIVDG ACLNHFTRIS NMIAKLAKTC TLRISPDKLN FILCDKLANG GVSMWCELEQ ENFFNEFQME GVSAENNEIY LELTSENLSR ALKTAQNARA LKIKLTNKHF PCLTVSVELL SMSSSSRIVT HDIPIKVIPR KLWKDLQEPV VPDPDVSIYL PVLKTMKSVV EKMKNISNHL VIEANLDGEL NLKIETELVC VTTHFKDLGN PPLASESTHE DRNVEHMAEV HIDIRKLLQF LAGQQVNPTK ALCNIVNNKM VHFDLLHEDV SLQYFIPALS