Recombinant humaan Hyaluronidase 1/HYAL1-eiwit

HYAL1, also known as Hyaluronidase-1, is a member of the endolytic glycoside hydrolase family. Human hyaluronidases (HYALs) are a group of five endo­β­N­acetyl­hexosaminidases that include HYAL1, HYAL2, HYAL3, HYAL4, and SPAM1 (PH20) . HYAL1 preferentially degrades hyaluronic acid present in the extracellular matrix of somatic tissues. This protein is active at an acidic pH and is the major hyaluronidase in plasma. Defects in HYAL1 are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. It is implicated in several types of cancers, likely due to the angiogenic effects of HYAL1-cleaved hyaluronan fragments. Recombinant human HYAL1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Hyaluronidase-1, Hyal-1, Hyaluronoglucosaminidase-1, Lung carcinoma protein 1, LuCa-1, HYAL1, Hyaluronidase 1, Hyaluronoglucosaminidase 1, Hyaluronoglucosaminidase1, LUCA 1, MPS9, NAT6, Plasma hyaluronidase, Tumor suppressor LUCA 1

HEK293

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

46.9kDa (420aa)

, ATGP3941-10 µg, ATGP3941-20 µg, ATGP3941-50 µg, ATGP3941-100 µg, ATGP3941-250 µg, ATGP3941-500 µg, ATGP3941-1 mg, ATGP3941-10, ATGP3941-20, ATGP3941-50, ATGP3941-100, ATGP3941-250, ATGP3941-500, ATGP3941-1

Csoka, A.B. et al. (2001) Matrix Biol. 20:499-508.; ; Jedrzejas, M. J. and Stern, R. (2005) Proteins 61:227-238.; ; Frost, GI. et al. (1997) Biochem. Biophys. Res. Commun. 236:10-15.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.

Signal Transduction, Cytoskeleton

NP_149349

Q12794

Human

Phe12