Recombinant humaan HYKK-eiwit

HYKK belongs to the aminoglycoside phosphotransferase family. This protein catalyzes the GTP-dependent phosphorylation of 5-hydroxy-L-lysine. Recombinant human HYKK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Hydroxylysine kinase, AGPHD1, Aminoglycoside phosphotransferase domain-containing protein 1, LOC123688

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

44.3 kDa (396aa) confirmed by MALDI-TOF

HYKK, Hydroxylysine kinase, AGPHD1, Aminoglycoside phosphotransferase domain-containing protein 1, LOC123688, ATGP2854-10 µg, ATGP2854-20 µg, ATGP2854-50 µg, ATGP2854-100 µg, ATGP2854-250 µg, ATGP2854-500 µg, ATGP2854-1 mg, ATGP2854-10, ATGP2854-20, ATGP2854-50, ATGP2854-100, ATGP2854-250, ATGP2854-500, ATGP2854-1

Veiga-da-Cunha, M., et al. (2012) J. Biol. Chem. 287 (10), 7246-7255

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 40% glycerol, 1mM DTT, 0.1mM PMSF

Other

NP_001013641

A2RU49

Human

MSSGNYQ QSEALSKPTF SEEQASALVE SVFGLKVSKV RPLPSYDDQN FHVYVSKTKD GPTEYVLKIS NTKASKNPDL IEVQNHIIMF LKAAGFPTAS VCHTKGDNTA SLVSVDSGSE IKSYLVRLLT YLPGRPIAEL PVSPQLLYEI GKLAAKLDKT LQRFHHPKLS SLHRENFIWN LKNVPLLEKY LYALGQNRNR EIVEHVIHLF KEEVMTKLSH FRECINHGDL NDHNILIESS KSASGNAEYQ VSGILDFGDM SYGYYVFEVA ITIMYMMIES KSPIQVGGHV LAGFESITPL TAVEKGALFL LVCSRFCQSL VMAAYSCQLY PENKDYLMVT AKTGWKHLQQ MFDMGQKAVE EIWFETAKSY ESGISM