Recombinant humaan I-TAC/CXCL11-eiwit

Chemokine (C-X-C motif) ligand 11 (CXCL11) is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant (I-TAC) and Interferon-gamma-inducible protein 9 (IP-9) . CXCL11 is strongly induced by IFN-gamma and IFN-beta, and weakly induced by IFN-alpha. It is chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. It is highly expressed in peripheral blood leukocytes, pancreas and liver, with moderate levels in thymus, spleen and lung and low expression levels were in small intestine, placenta and prostate. Recombinant human CXCL11 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

C-X-C motif chemokine ligand 11, C-X-C motif chemokine 11, Beta-R1, H174, Interferon gamma-inducible protein 9, IP-9, Interferon-inducible T-cell alpha chemoattractant, I-TAC, Small-inducible cytokine B11, SCYB11, SCYB9B, b-R1, IP-9

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

10.6 kDa (94aa) confirmed by MALDI-TOF

CXCL11, I-TAC, C-X-C motif chemokine 11, b-R1, H174, I-TAC, IP-9, IP9, MGC102770, SCYB11, SCYB9B, ATGP1296-10 µg, ATGP1296-20 µg, ATGP1296-50 µg, ATGP1296-100 µg, ATGP1296-250 µg, ATGP1296-500 µg, ATGP1296-1 mg, ATGP1296-10, ATGP1296-20, ATGP1296-50, ATGP1296-100, ATGP1296-250, ATGP1296-500, ATGP1296-1

Cole KE, et al. (1998) J. Exp. Med. 187 (12) : 2009-21. ; ; Mach, F., et al. (1999) J. Clin. Invest. 104: 1041-1050.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 10 mM Sodium Citrate buffer (pH 3.5) containing 2mM DTT, 20% glycerol

Hormones & Cytokines

NP_005400

O14625

Human

FPMFKRGRC LCIGPGVKAV KVADIEKASI MYPSNNCDKI EVIITLKENK GQRCLNPKSK QARLIIKKVE RKNF