Recombinant humaan ICAM-3/CD50-eiwit

ICAM-3/CD50, also known as CD50 (Cluster of Differentiation 50), is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leukocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signaling molecule. Recombinant human ICAM-3/CD50, fused hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human ICAM-3/CD50 stimulates cell adhesion of the HL-60 human promyelocytic cells. The ED50 range ≤4 µg/mL.

ICAM3, Intercellular adhesion molecule 3, ICAM-3, CD50, CDW50, ICAM-R, intercellular adhesion molecule 3 isoform 1

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of HL-60 human promyelocytic cells. When cells are added to Human ICAM-3/CD50 coated plates, the ED50 range ≤4 µg/mL.

76.7 kDa (698aa)

ICAM3, CD50, ATGP3931-10 µg, ATGP3931-20 µg, ATGP3931-50 µg, ATGP3931-100 µg, ATGP3931-250 µg, ATGP3931-500 µg, ATGP3931-1 mg, ATGP3931-10, ATGP3931-20, ATGP3931-50, ATGP3931-100, ATGP3931-250, ATGP3931-500, ATGP3931-1

De Fougerolles AR., et al, (1992) J Exp Med. 175:185-190.; ; Serrador JM., et al, (1997) J Cell Biol. 138:1409-1423.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_002153.2

P32942

Human

Ala

QEFLLRV EPQNPVLSAG GSLFVNCSTD CPSSEKIALE TSLSKELVAS GMGWAAFNLS NVTGNSRILC SVYCNGSQIT GSSNITVYRL PERVELAPLP PWQPVGQNFT LRCQVEDGSP RTSLTVVLLR WEEELSRQPA VEEPAEVTAT VLASRDDHGA PFSCRTELDM QPQGLGLFVN TSAPRQLRTF VLPVTPPRLV APRFLEVETS WPVDCTLDGL FPASEAQVYL ALGDQMLNAT VMNHGDTLTA TATATARADQ EGAREIVCNV TLGGERREAR ENLTVFSFLG PIVNLSEPTA HEGSTVTVSC MAGARVQVTL DGVPAAAPGQ PAQLQLNATE SDDGRSFFCS ATLEVDGEFL HRNSSVQLRV LYGPKIDRAT CPQHLKWKDK TRHVLQCQAR GNPYPELRCL KEGSSREVPV GIPFFVNVTH NGTYQCQASS SRGKYTLVVV MDIEAGSSH