Recombinant humaan ICOS-eiwit

ICOS, also known as inducible T-cell costimulator, is a member of the CD28 family of immune-assisted stimulatory receptors. The interaction of B7-H2 / ICOS plays a crucial role in Th cell differentiation, T-B cell interaction and humoral immune response essential for the formation of reproductive centers and the production of cytokine IL-4. In addition, this protein is more potent in inducing IL-10 production, a cytokine important for the inhibitory function of T regulatory cells. The B7-1 / B7-2-CD28 / CTLA-4 and ICOS-B7RP-1 pathways provide an important second signal that can regulate the activation, inhibition and fine regulation of T-lymphocyte responses. It stimulates production of Th1 and Th2 cytokines, but may play a role in the generation of Th2 cells. Recombinant human ICOS, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Inducible T-cell costimulator, ICOS, AILIM, CD278, CVID1

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

40.8 kDa (362aa)

ICOS, Inducible T-cell costimulator, ICOS, AILIM, CD278, CVID1, ATGP3771-10 µg, ATGP3771-20 µg, ATGP3771-50 µg, ATGP3771-100 µg, ATGP3771-250 µg, ATGP3771-500 µg, ATGP3771-1 mg, ATGP3771-10, ATGP3771-20, ATGP3771-50, ATGP3771-100, ATGP3771-250, ATGP3771-500, ATGP3771-1

Gonzalo, J.A., et al. (2001) J. Immunol. 166:1-5.; ; Tezukak., et al. (2000) Biochem Biophys Res Commun. 276:335-345

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM MES buffer (pH 5.5) containing 40% glycerol, 2mM DTT, 1mM EDTA 0.1M NaCl

Immunology

NP_036224

Q9Y6W8

Human

Ala

EINGSAN YEMFIFHNGG VQILCKYPDI VQQFKMQLLK GGQILCDLTK TKGSGNTVSI KSLKFCHSQL SNNSVSFFLY NLDHSHANYY FCNLSIFDPP PFKVTLTGGY LHIYESQLCC QLK