Recombinant humaan ICT1-eiwit

Peptidyl-tRNA hydrolase ICT1 acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. It may be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. Recombinant human ICT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Peptidyl-tRNA hydrolase ICT1 mitochondrial, Peptidyl-tRNA hydrolase ICT1, mitochondrial, DS-1, DS1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

22.8 kDa (200aa) confirmed by MALDI-TOF

ICT1, Peptidyl-tRNA hydrolase ICT1 mitochondrial, Peptidyl-tRNA hydrolase ICT1, mitochondrial, DS-1, DS1, ATGP1834-10 µg, ATGP1834-20 µg, ATGP1834-50 µg, ATGP1834-100 µg, ATGP1834-250 µg, ATGP1834-500 µg, ATGP1834-1 mg, ATGP1834-10, ATGP1834-20, ATGP1834-50, ATGP1834-100, ATGP1834-250, ATGP1834-500, ATGP1834-1

Richter R., et al. (2010) EMBO J. 29:1116-1125; ; van Belzen N., et al. (1995) Eur. J. Biochem. 234:843-848;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 1mM DTT

Cancer

NP_001536

Q14197

Human

MGSSHHHHHH SSGLVPRGSH MGSLHKQKDG TEFKSIYSLD KLYPESQGSD TAWRVPNGAK QADSDIPLDR LTISYCRSSG PGGQNVNKVN SKAEVRFHLA TAEWIAEPVR QKIAITHKNK INRLGELILT SESSRYQFRN LADCLQKIRD MITEASQTPK EPTKEDVKLH RIRIENMNRE RLRQKRIHSA VKTSRRVDMD