Recombinant humaan IDH3G-eiwit

IDH3G, also known as isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, belongs to the isocitrate and isopropylmalate dehydrogenases family. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH3G is a gamma subunit of one isozyme of isocitrate dehydrogenase that belongs to a distinct subclass, which utilizes NAD (+) as the electron acceptor, and is localised to the mitochondrial matrix. Recombinant human IDH3G protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Isocitrate dehydrogenase [NAD] subunit gamma mitochondrial, Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, H-IDHG

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

41.1 kDa (375aa) confirmed by MALDI-TOF

IDH3G, Isocitrate dehydrogenase [NAD] subunit gamma mitochondrial, Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, H-IDHG, ATGP1324-10 µg, ATGP1324-20 µg, ATGP1324-50 µg, ATGP1324-100 µg, ATGP1324-250 µg, ATGP1324-500 µg, ATGP1324-1 mg, ATGP1324-10, ATGP1324-20, ATGP1324-50, ATGP1324-100, ATGP1324-250, ATGP1324-500, ATGP1324-1

Kim Y.-O., et al. (1999) J. Biol. Chem. 274:36866-36875; ; Brenner V., et al. (1997) Genomics. 44:8-14

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl, 5mM DTT, 2mM EDTA

Metabolism

NP_004126

P51553

Human

FSEQTIPPS AKYGGRHTVT MIPGDGIGPE LMLHVKSVFR HACVPVDFEE VHVSSNADEE DIRNAIMAIR RNRVALKGNI ETNHNLPPSH KSRNNILRTS LDLYANVIHC KSLPGVVTRH KDIDILIVRE NTEGEYSSLE HESVAGVVES LKIITKAKSL RIAEYAFKLA QESGRKKVTA VHKANIMKLG DGLFLQCCRE VAARYPQITF ENMIVDNTTM QLVSRPQQFD VMVMPNLYGN IVNNVCAGLV GGPGLVAGAN YGHVYAVFET ATRNTGKSIA NKNIANPTAT LLASCMMLDH LKLHSYATSI RKAVLASMDN ENMHTPDIGG QGTTSEAIQD VIRHIRVING RAVEA