Recombinant humaan IDI1-eiwit

Isopentenyl-diphosphate isomerase 1, also known as IDI1, is a member of the IPP isomerase type I family and is involved in cholesterol biosynthesis. IDI1 is a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which is the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. Recombinant human IDI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Isopentenyl-diphosphate isomerase 1, IPP1, IPPI1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.6 kDa (248aa) confirmed by MALDI-TOF

IDI1, Isopentenyl-diphosphate isomerase 1, IPP1, IPPI1, ATGP1277-10 µg, ATGP1277-20 µg, ATGP1277-50 µg, ATGP1277-100 µg, ATGP1277-250 µg, ATGP1277-500 µg, ATGP1277-1 mg, ATGP1277-10, ATGP1277-20, ATGP1277-50, ATGP1277-100, ATGP1277-250, ATGP1277-500, ATGP1277-1

Hahn F M., et al. (1996) Arch Biochem Biophys. 332:30-34.; ; Zheng W., et al. (2007) J Mol Biol. 366:1447-1458.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. PBS containing 20% glycerol, 0.1mM PMSF

Metabolism

AAH57827

Q13907

Human

MMPEINTNHL DKQQVQLLAE MCILIDENDN KIGAETKKNC HLNENIEKGL LHRAFSVFLF NTENKLLLQQ RSDAKITFPG CFTNTCCSHP LSNPAELEES DALGVRRAAQ RRLKAELGIP LEEVPPEEIN YLTRIHYKAQ SDGIWGEHEI DYILLVRKNV TLNPDPNEIK SYCYVSKEEL KELLKKAASG EIKITPWFKI IAATFLFKWW DNLNHLNQFV DHEKIYRM