Recombinant humaan IDI2-eiwit

IDI2, also known as isopentenyl-diphosphate delta-isomerase 2, belongs to the IPP isomerase type 1 family. IDI2 catalyzes the 1, 3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP) . Recombinant human IDI2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Isopentenyl-diphosphate delta-isomerase 2, IPPI2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

28.9 kDa (247aa) confirmed by MALDI-TOF

IDI2, Isopentenyl-diphosphate delta-isomerase 2, IPPI2, ATGP1000-10 µg, ATGP1000-20 µg, ATGP1000-50 µg, ATGP1000-100 µg, ATGP1000-250 µg, ATGP1000-500 µg, ATGP1000-1 mg, ATGP1000-10, ATGP1000-20, ATGP1000-50, ATGP1000-100, ATGP1000-250, ATGP1000-500, ATGP1000-1

Clizbe D.B., et al. (2007) Biol Chem. 282:6668-6676; ; Ota T., et al. (2004) Genet. 36:40-45

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1mM PMSF

Metabolism

NP_150286

Q9BXS1

Human

MSDINLDWVD RRQLQRLEEM LIVVDENDKV IGADTKRNCH LNENIEKGLL HRAFSVVLFN TKNRILIQQR SDTKVTFPGY FTDSCSSHPL YNPAELEEKD AIGVRRAAQR RLQAELGIPG EQISPEDIVF MTIYHHKAKS DRIWGEHEIC YLLLVRKNVT LNPDPSETKS ILYLSQEELW ELLEREARGE VKVTPWLRTI AERFLYRWWP HLDDVTPFVE LHKIHRV