Recombinant humaan IDO-eiwit

IDO1 is an indoleamine 2, 3-dioxygenase - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, and serotonin. It is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan. Recombinant human IDO1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of L-Tryptophan to N-formyl-L-kynurenine per minute at pH 6.5 at 25C.

Indoleamine 2,3-dioxygenase 1, IDO, IDO-1, INDO

E.coli

Human

His-Tag

Enzyme Activity, SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 300pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of L-Tryptophan to N-formyl-L-kynurenine per minute at pH 6.5 at 25C.

47.7 kDa (426aa) confirmed by MALDI-TOF

IDO, Indoleamine 2,3-dioxygenase 1, IDO, IDO-1, INDO, ATGP3488-10 µg, ATGP3488-20 µg, ATGP3488-50 µg, ATGP3488-100 µg, ATGP3488-250 µg, ATGP3488-500 µg, ATGP3488-1 mg, ATGP3488-10, ATGP3488-20, ATGP3488-50, ATGP3488-100, ATGP3488-250, ATGP3488-500, ATGP3488-1

Metz R., et al (2007) . Nature Cancer Res. 67:7082-7087; ; Yuasa H.J., et al (2007) . J. Mol. Evol. 65:705-714

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Metabolism

NP_002155

P14902

Human

MGSSHHHHHH SSGLVPRGSH MGSMAHAMEN SWTISKEYHI DEEVGFALPN PQENLPDFYN DWMFIAKHLP DLIESGQLRE RVEKLNMLSI DHLTDHKSQR LARLVLGCIT MAYVWGKGHG DVRKVLPRNI AVPYCQLSKK LELPPILVYA DCVLANWKKK DPNKPLTYEN MDVLFSFRDG DCSKGFFLVS LLVEIAAASA IKVIPTVFKA MQMQERDTLL KALLEIASCL EKALQVFHQI HDHVNPKAFF SVLRIYLSGW KGNPQLSDGL VYEGFWEDPK EFAGGSAGQS SVFQCFDVLL GIQQTAGGGH AAQFLQDMRR YMPPAHRNFL CSLESNPSVR EFVLSKGDAG LREAYDACVK ALVSLRSYHL QIVTKYILIP ASQQPKENKT SEDPSKLEAK GTGGTDLMNF LKTVRSTTEK SLLKEG