Recombinant humaan Iduronaat 2-Sulfatase-eiwit

IDS, as known as iduronate 2-sulfatase isoform, is a member of the highly conserved sulfatase family of enzymes that catalyze the hydrolysis of O-sulfate and N-salfate esters from a varity of substrates. This protein is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and dermatan sulfate. It hydrolyzes the 2-sulfate group of the IDS units of the GAG. Recombinant human IDS, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Iduronate 2-sulfatase isoform, IDS, MPS2, SIDS

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

60.3 kDa (533aa)

Iduronate2-Sulfatase, ATGP3462-10 µg, ATGP3462-20 µg, ATGP3462-50 µg, ATGP3462-100 µg, ATGP3462-250 µg, ATGP3462-500 µg, ATGP3462-1 mg, ATGP3462-10, ATGP3462-20, ATGP3462-50, ATGP3462-100, ATGP3462-250, ATGP3462-500, ATGP3462-1

Torres LC., et al, (2014) Clin. Immunol. 154:100-104.; ; Chistiakov DA., et al, (2014) J. Genet. Genomics. 41:197-203.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Enzymes & Proteases

NP_000193

P22304

Human

Ser26