Recombinant humaan IkB-alfa eiwit

NF-kappa-B inhibitor alpha, also known as NFKBIA, is members of the IkB family of proteins can be divided into four groups (IkB-alpha, IkB-beta, IkB-gamma, IkB-epsilon) . NFKBIA inhibits the NFkB complex by binding and sequestering it in the cytoplasm. upon stimulation, NFKBIA is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Recombinant human NFKBIA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NF-kappa-B inhibitor alpha, IKBA, MAD-3, NFKBI

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

37.7 kDa (337aa) confirmed by MALDI-TOF

IkB-a, NF-kappa-B inhibitor alpha, IKBA, MAD-3, NFKBI, ATGP1261-10 µg, ATGP1261-20 µg, ATGP1261-50 µg, ATGP1261-100 µg, ATGP1261-250 µg, ATGP1261-500 µg, ATGP1261-1 mg, ATGP1261-10, ATGP1261-20, ATGP1261-50, ATGP1261-100, ATGP1261-250, ATGP1261-500, ATGP1261-1

Simson C S., et al. (2000) J Biol Chem. 275:16879-16884.; ; Singh S., et al. (1996) J Biol Chem. 271:31049-31054.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl

Signal Transduction

NP_065390.1

P25963

Human

MFQAAERPQE WAMEGPRDGL KKERLLDDRH DSGLDSMKDE EYEQMVKELQ EIRLEPQEVP RGSEPWKQQL TEDGDSFLHL AIIHEEKALT MEVIRQVKGD LAFLNFQNNL QQTPLHLAVI TNQPEIAEAL LGAGCDPELR DFRGNTPLHL ACEQGCLASV GVLTQSCTTP HLHSILKATN YNGHTCLHLA SIHGYLGIVE LLVSLGADVN AQEPCNGRTA LHLAVDLQNP DLVSLLLKCG ADVNRVTYQG YSPYQLTWGR PSTRIQQQLG QLTLENLQML PESEDEESYD TESEFTEFTE DELPYDDCVF GGQRLTL