Recombinant humaan IkB-bèta-eiwit

NFKBIB, also known as NF-kappa-B inhibitor beta, belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Recombinant human NFKBIB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

NF-kappa-B inhibitor beta, IKBB, TRIP9

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

40.3 kDa (380aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

IkB-b, NF-kappa-B inhibitor beta, IKBB, TRIP9, ATGP1428-10 µg, ATGP1428-20 µg, ATGP1428-50 µg, ATGP1428-100 µg, ATGP1428-250 µg, ATGP1428-500 µg, ATGP1428-1 mg, ATGP1428-10, ATGP1428-20, ATGP1428-50, ATGP1428-100, ATGP1428-250, ATGP1428-500, ATGP1428-1

Fenwick C., et al. (2000) Science. 287:869-873; ; DiDonato J.A., et al. (1996) Mol. Cell. Biol. 16:1295-1304

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Signal Transduction

NP_002494

Q15653

Human

MGSSHHHHHH SSGLVPRGSH MGSHMAGVAC LGKAADADEW CDSGLGSLGP DAAAPGGPGL GAELGPGLSW APLVFGYVTE DGDTALHLAV IHQHEPFLDF LLGFSAGTEY MDLQNDLGQT ALHLAAILGE TSTVEKLYAA GAGLCVAERR GHTALHLACR VGAHACARAL LQPRPRRPRE APDTYLAQGP DRTPDTNHTP VALYPDSDLE KEEEESEEDW KLQLEAENYE GHTPLHVAVI HKDVEMVRLL RDAGADLDKP EPTCGRSPLH LAVEAQAADV LELLLRAGAN PAARMYGGRT PLGSAMLRPN PILARLLRAH GAPEPEGEDE KSGPCSSSSD SDSGDEGDEY DDIVVHSSRS QTRLPPTPAS KPLPDDPRPV