Recombinant humaan IL-1 alfa/IL1A-eiwit

IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. IL-1alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. Recombinant human IL-1alpha was expressed in E.coli and purified by using conventional chromatography techniques.

Human IL-1 alpha /IL-1F1 stimulates cell proliferation of the D10.G4.1 mouse helper T cell. The ED50 range is less or equal to 0.04 ng/mL.

Interleukin 1 alpha, preinterleukin 1 alpha, Hematopoietin-1, Pro-interleukin-1-alpha, IL1F1, IL-1A, IL1-ALPHA, IL1

E.coli

Human

His-Tag

SDS-PAGE, Bioactivity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 for this effect is less or equal to 0.04 ng/mL.

22.4 kDa (197aa) confirmed by MALDI-TOF

IL1 a , IL1F1, ATGP3864-10 µg, ATGP3864-20 µg, ATGP3864-50 µg, ATGP3864-100 µg, ATGP3864-250 µg, ATGP3864-500 µg, ATGP3864-1 mg, ATGP3864-10, ATGP3864-20, ATGP3864-50, ATGP3864-100, ATGP3864-250, ATGP3864-500, ATGP3864-1

Misener VL., et al. (2008) . Neuropsychobiology. 58 (2) :71-80.; ; Sarkar O., et al. (2008) . Biol Reprod. 78 (3) :445-54.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Hormones & Cytokines

NP_000566

P01583

Human

SA PFSFLSNVKY NFMRIIKYEF ILNDALNQSI IRANDQYLTA AALHNLDEAV KFDMGAYKSS KDDAKITVIL RISKTQLYVT AQDEDQPVLL KEMPEIPKTI TGSETNLLFF WETHGTKNYF TSVAHPNLFI ATKQDYWVCL AGGPPSITDF QILENQA