Recombinant humaan IL-10R alfa/IL10RA-eiwit

IL-10 R alpha, also known as Interleukin-10 receptor subunit alpha, is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. It has been shown to interact with Interleukin 10 and Janus kinase 1. Recombinant human IL-10 R alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human IL-10 R alpha inhibits Human IL-10 induced cell proliferation in the MC/9 mouse mast cells. The ED50 range is ≤300 ng/mL.

Interleukin 10 receptor subunit alpha, IL-10 receptor subunit alpha, IL-10R subunit alpha, IL-10RA, CD210, CDw210a, Interleukin-10 receptor subunit 1, IL-10R subunit 1, IL-10R1, HIL-10R

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.2 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its ability to inhibit proliferation using MC/9 mouse mast cells. The ED50 for this effect is ≤300 ng/mL with Human IL-10.

25.2 kDa (220aa)

IL10 R a, ATGP3910-10 µg, ATGP3910-20 µg, ATGP3910-50 µg, ATGP3910-100 µg, ATGP3910-250 µg, ATGP3910-500 µg, ATGP3910-1 mg, ATGP3910-10, ATGP3910-20, ATGP3910-50, ATGP3910-100, ATGP3910-250, ATGP3910-500, ATGP3910-1

Josephson K, et al. (2001) Acta Crystallogr D Biol Crystallogr. 57 (Pt 12) :1908-11.; ; Usacheva A, etal. (2002) J Immunol. 169 (3) :1302-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Hormones & Cytokines

NP_001549

Q13651

Human

His22