Recombinant humaan IL-12 p70 eiwit

IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 kD subunit. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. IL12 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Recombinant human IL12 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.

Human IL12 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ≤1 ng/mL.

IL12A, IL12B, CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, Interleukin 12 alpha chain, Interleukin-12 beta chain, NK cell stimulatory factor chain 1, p35, NK cell stimulatory factor chain 2, p40, NKSF1, NKSF2

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤1 ng/mL.

34.6 kDa (p40, 306aa) ; ;23.3 kDa (p35, 203aa) ; ; The heterodimeric IL12 has a predicted Molecular weight of 57.9 kDa (509aa) . Molecular weight on SDS-PAGE will appear higher.

IL12, ATGP2843-10 µg, ATGP2843-20 µg, ATGP2843-50 µg, ATGP2843-100 µg, ATGP2843-250 µg, ATGP2843-500 µg, ATGP2843-1 mg, ATGP2843-10, ATGP2843-20, ATGP2843-50, ATGP2843-100, ATGP2843-250, ATGP2843-500, ATGP2843-1

Barton J.L., et al. (2000) Eur. J. Immunol. 30:3299-3308; ; Bazan J.F., et al. (2001) J. Immunol. 167:1440-1446

Kim HR, et al. Anti-cancer activity and mechanistic features of a NK cell activating molecule. (Cancer Immunol Immunother. 2009) {https://pubmed.ncbi.nlm.nih.gov/19259669/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Hormones & Cytokines

NP_002178 (p40) AAK84425 (p35)

P29460 (p40) /P29459 (p35)

Human

Met1