Recombinant humaan IL-15R alfa/IL15RA eiwit

IL15RA, also known as interleukin-15 receptor subunit alpha isoform 1, is a high-affinity receptor for interleukin-15. It associates as a heterotrimer with the IL-2 receptor beta and gamma subunits to initiate signal transduction. This protein can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. IL15/IL15R alpha complexes can transmit reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL 15/IL15R alpha expressing cells. Recombinant human IL15RA protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human IL-15 R alpha inhibits Human IL-15 induced cell proliferation in the CTLL2 mouse cytotoxic T cells. The ED50 range ≤2 ng/mL

Interleukin-15 receptor subunit alpha isoform 1, IL15RA, CD215, IL-15 receptor subunit alpha

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its ability to inhibit proliferation using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is ≤2 ng/mL with Human IL-15.

45.6 kDa (417aa)

IL15 R a, ATGP3672-10 µg, ATGP3672-20 µg, ATGP3672-50 µg, ATGP3672-100 µg, ATGP3672-250 µg, ATGP3672-500 µg, ATGP3672-1 mg, ATGP3672-10, ATGP3672-20, ATGP3672-50, ATGP3672-100, ATGP3672-250, ATGP3672-500, ATGP3672-1

Epardaud M., et al, (2008) Cancer Res. 68:2972-2983.; ; Stoklasek TA., et al, (2006) J Immunol. 177:6072-6080.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Apoptosis

NP_002180

Q13261

Human

Ala

ITCPPPM SVEHADIWVK SYSLYSRERY ICNSGFKRKA GTSSLTECVL NKATNVAHWT TPSLKCIRDP ALVHQRPAPP STVTTAGVTP QPESLSPSGK EPAASSPSSN NTAATTAAIV PGSQLMPSKS PSTGTTEISS HESSHGTPSQ TTAKNWELTA SASHQPPGVY PQGHSDTT