Recombinant humaan IL-17A-eiwit

IL17A, also known as Interleukin 17, is a cytokine that acts as a potent mediator in delayed-type reactions by increasing chemokine production in various tissues to recruit monocytes and neutrophils to the site of inflammation. It is produced by T-helper cells and is induced by IL23 which results in destructive tissue damage in delayed-type reactions. This protein is a 155 amino acid protein that is a disulfide-linked, homodimeric, secreted glycoprotein with a molecular mass of 35kDa. The structure of IL17A consists of a signal peptide of 23 amino acids followed by a 123 aa chain region characteristic of the IL17 family. An N-linked glycosylation site on the protein was first identified after purification of the protein revealed two bands, one at 15kDa and another at 20kDa. Recombinant human IL17A, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human IL-17A stimulates IL-6 secretion of the NIH/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 7 ng/mL.

Interleukin 17A, IL-17, IL-17A, IL17, CTLA8, Cytotoxic T-lymphocyte-associated protein 8, Cytotoxic T-lymphocyte-associated serine esterase 8

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

The activity is determined by the IL-6 ELISA in a using NIH/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 7 ng/mL.

15.9 kDa (138aa)

IL17A, Interleukin 17A, CTLA8, IL-17, IL-17A, IL17, ATGP3083-10 µg, ATGP3083-20 µg, ATGP3083-50 µg, ATGP3083-100 µg, ATGP3083-250 µg, ATGP3083-500 µg, ATGP3083-1 mg, ATGP3083-10, ATGP3083-20, ATGP3083-50, ATGP3083-100, ATGP3083-250, ATGP3083-500, ATGP3083-1

Yao Z, et al (1995) J Immunol. 155 (12) :5483-6.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM EDTA, 0.1mM PMSF

Hormones & Cytokines

NP_002181.1

Q16552

Human

Gly24