Recombinant humaan IL-1R bèta/IL1R2-eiwit

IL1R2, also known as interleukin-1 receptor type 2 isoform 1, is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein is expressed predominantly by, T cells, fibroblasts and endothelial cells. It binds interleukin-1 alpha (IL1A), interleukin-1beta (IL1B), and interleukin 1 receptor antagonist (IL1RN), preventing them from binding to their regular receptors and thereby inhibiting the transduction of their signaling. Also, Interleukin-4 (IL4) is reported to antagonize the activity of interleukin-1 by inducing the expression and release of this cytokine. Recombinant human IL1R2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 range ≤400 ng/mL with Human IL-1 beta/IL-1F2.

Interleukin-1 receptor type 2, CD121 antigen-like family member B, CDw121b, IL-1 type II receptor, Interleukin-1 receptor beta, IL-1R-beta, Interleukin-1 receptor type II, CD121b, IL1RB

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 range ≤400 ng/mL with Human IL-1 beta/IL-1F2.

38.8 kDa (338aa)

IL1 RII, ATGP3733-10 µg, ATGP3733-20 µg, ATGP3733-50 µg, ATGP3733-100 µg, ATGP3733-250 µg, ATGP3733-500 µg, ATGP3733-1 mg, ATGP3733-10, ATGP3733-20, ATGP3733-50, ATGP3733-100, ATGP3733-250, ATGP3733-500, ATGP3733-1

Greenfeder SA., et al, (1995) J Biol Chem 270:13757-13765.; ; Granowitz EV., et al, (1991) J. Biol. Chem. 266:14147-14150.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Hormones & Cytokines

NP_004624.1

P27930

Human

Phe14