Recombinant humaan IL-21-eiwit

IL21, also known as interleukin-21 isoform 1, is a T-helper cytokine that regulates humoral immunity and cell-mediated anti-tumour responses. It has broad immunoregulatory activity and can modulate both humoral and cell-mediated responses. It is important in the regulation of haematopoiesis and immune responses, and can influence lymphocyte development. It is produced by activated T cells, and it influences proliferation of T and B cells and cytolytic activity of natural killer cells. It may be a critical factor in the control of persistent viral infections. It has a potential predicating significance for survival time in patients in the development of sporadic CRC. It binds to the IL-21R and can activate Janus kinase JAK1, JAK3, STAT1, and STAT3 in EDgamma-16 cells. Recombinant human IL21, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

The activity is determined by the IFN-g ELISA in a using NK-92 cell. The ED50 range ≤ 10 ng/mL.

Interleukin-21 isoform 1, IL21, CVID11, IL-21, Za11

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

The activity is determined by the IFN-g ELISA in a using NK-92 cell. The ED50 range ≤ 10 ng/mL.

16.9 kDa (145aa)

IL21, Interleukin-21 isoform 1, IL21, CVID11, IL-21, Za11, ATGP3802-10 µg, ATGP3802-20 µg, ATGP3802-50 µg, ATGP3802-100 µg, ATGP3802-250 µg, ATGP3802-500 µg, ATGP3802-1 mg, ATGP3802-10, ATGP3802-20, ATGP3802-50, ATGP3802-100, ATGP3802-250, ATGP3802-500, ATGP3802-1

Parrish-Novak J., et al. (2000) Nature. 408:57-63.; ; Cui G., et al. (2017) Clin Immunol. 183:266-272.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Immunology

NP_068575

Q9HBE4

Human

Ala

QGQD RHMIRMRQLI DIVDQLKNYV NDLVPEFLPA PEDVETNCEW SAFSCFQKAQ LKSANTGNNE RIINVSIKKL KRKPPSTNAG RRQKHRLTCP SCDSYEKKPP KEFLERFKSL LQKMIHQHLS SRTHGSEDS