Recombinant humaan IL-23 eiwit

IL23 p40 and p19, also known as interleukin-12 subunit beta/interleukin-23 subunit alpha, is a heterodimeric cytokine composed of two disulfide-linked subunit, a p19 subunit that is unique to IL23, and a p40 subunit that is shared with IL12. The p19 subunit has homology to the p35 subunit of IL12, as well as to other single chain sytokines sub as IL6 and IL11. It is produced by macrophages and B lymphocytes and has multiple effects on T-cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFN-gamma and TNF production. Recombinant human IL23 p40 and p19, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Interleukin-12 subunit beta / Interleukin-23 subunit alpha, CLMF, CLMF2, IL-12B, IMD28, IMD29, NKSF, NKSF2/ IL-23, IL-23A, IL23P19, P19, SGRF

Baculovirus

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

34.6 kDa (p40, 306aa) ; ;19.5 kDa (p19, 176aa)

IL23, ATGP3598-10 µg, ATGP3598-20 µg, ATGP3598-50 µg, ATGP3598-100 µg, ATGP3598-250 µg, ATGP3598-500 µg, ATGP3598-1 mg, ATGP3598-10, ATGP3598-20, ATGP3598-50, ATGP3598-100, ATGP3598-250, ATGP3598-500, ATGP3598-1

Oppmann B., et al, (2000) Immunity. 13:715-25.; ; Parham C., et al, (2002) J. lmmunol. 168:5699-708.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Hormones & Cytokines

NP_002178 (p40) NP_057668 (p19)

P29460 (p40) /Q9NPF7 (p19)

Human

Ile23, Arg20