Recombinant humaan IL-3 eiwit

IL3, as known as interleukin 3, is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. Also, this protein affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulation factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factors and WEHI-3 growth factor. Recombinant human IL3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤0.2 ng/mL.

IL-3, Multipotential colony-stimulating factor (multi-CSF), Mast cell growth factor (MCGF), Interleukin 3 (colony-stimulating factor, multiple), MuLTI-CSF, MCGF, Multilineage-colony-stimulating factor, Hematopoietic growth factor, P-cell stimulating factor, Mast-cell growth factor

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤0.2 ng/mL.

16.1 kDa (141aa)

IL3, ATGP3365-10 µg, ATGP3365-20 µg, ATGP3365-50 µg, ATGP3365-100 µg, ATGP3365-250 µg, ATGP3365-500 µg, ATGP3365-1 mg, ATGP3365-10, ATGP3365-20, ATGP3365-50, ATGP3365-100, ATGP3365-250, ATGP3365-500, ATGP3365-1

Chavany, C., et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:11354-11359.; ; Otsuka, T., et al. (1988) J. Immunol. 140:2288-2295.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Hormones & Cytokines

NP_000579

P08700

Human

Ala20