Recombinant humaan IL-32 eiwit

Interleukin 32 isoform A belongs to cytokine family. This protein expression is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. It has the ability to induce proinflammatory cytokines such as TNFalpha and IL8 in THP1 cells, and activates typical cytokine signaling pathways involving NFkB and p38. IL-32 can also support osteoclast differentiation but not osteoclast activation by regulating the MAPK/ERK pathway and the actin cytoskeleton. Recombinant human Interleukin 32, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Interleukin 32, Natural killer cells protein 4, Natural killer cell transcript 4, NK4, Tumor necrosis factor alpha-inducing factor, TAIF, TAIFb, TAIFd

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

19.1 kDa (168aa) confirmed by MALDI-TOF

IL32 a, Interleukin 32 isoform A, IL-32, Natural killer cells protein 4 (NK4), Tumor necrosis factor alpha-inducing factor (TAIF), Interleukin 32, Interleukin 32 isoform A IL 32alpha, IL 32beta, IL 32delta, IL 32gamma, Interleukin 32, Natural killer cells protein 4, NK 4, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, il32, ATGP0266-10 µg, ATGP0266-20 µg, ATGP0266-50 µg, ATGP0266-100 µg, ATGP0266-250 µg, ATGP0266-500 µg, ATGP0266-1 mg, ATGP0266-10, ATGP0266-20, ATGP0266-50, ATGP0266-100, ATGP0266-250, ATGP0266-500, ATGP0266-1

Kim SH., et al. (2005) . Immunity. 22 (1) :131-42 ; ; Kobayashi H., et al. (2009) . Int J Radiat Oncol Biol Phys. 74 (5) :1573-9;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Hormones & Cytokines

NP_001012651.1

P24001

Human

MCF PKVLSDDMKK LKARMHQAIE RFYDKMQNAE SGRGQVMSSL AELEDDFKEG YLETVAAYYE EQHPELTPLL EKERDGLRCR GNRSPVPDVE DPATEEPGES FCDKSYGAPR GDKEELTPQK CSEPQSSK