Recombinant humaan IL-4 eiwit

IL-4 is a multi-functional cytokine that plays a critical role in the regulation of immune responses. It induces differentiation of naive helper T cells to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. IL-4 is produced primarily by mast cells, Th2 cells, eosinophils and basophils. It is closely related and has functions similar to interleukin 13. This protein has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL-4 plays a dominant role in the development of allergic inflammation and asthma. Also, it is crucial in the development of allergic airway inflammation originates. Recombinant human IL-4, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Human IL-4 in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 0.5 ng/mL.

Pitrakinra, MGC79402, Lymphocyte stimulatory factor 1, Interleukin-4 isoform 1, Interleukin 4, IL-4, BSF1, Binetrakin, BCGF1, B-cell stimulatory factor 1, B-cell growth factor 1, BCDF

HEK293

Mouse

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS - PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 0.5 ng/mL.

16.1kDa (139aa)

Pitrakinra, Pitrakinara, MGC79402, Lymphocyte stimulatory factor 1, Interleukin-4 isoform 1, Interleukin-4, Interleukin4, Interleukin 4 isoform 1, Interleukin 4, IL-4, IL4, IL 4, BSF-1, BSF1, BSF 1, Binetrakin, BCGF-1, BCGF1, BCGF 1, B-cell stimulatory factor 1, B-cell growth factor 1, BCDF, B cell stimulatory factor 1, B cell growth factor 1, ATGP3983-10 µg, ATGP3983-20 µg, ATGP3983-50 µg, ATGP3983-100 µg, ATGP3983-250 µg, ATGP3983-500 µg, ATGP3983-1 mg, ATGP3983-010, ATGP3983-020, ATGP3983-050, ATGP3983-100, ATGP3983-250, ATGP3983-500, ATGP3983-01M

Bhattarai P., et al, (2016) Cell Reports. 17: 941–948.; ; Cheng Ho ., et al, (2016) Adv Exp Med Biol. 941:31-77.; ; Marsha Wills-Karp., et al, (2008) Sci Signal. 1:pe55.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology, Hormones & Cytokines

NP_000580

P05112

Mouse

Asp

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