Recombinant humaan IL-5 eiwit

IL5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. It related hematopoietic cytokines that are important for allergic inflammation. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. Recombinant human IL5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤1.5 ng/mL.

Interleukin-5, IL-5, EDF, TRF, T-cell replacing factor, B cell differentiation factor I, Eosinophil differentiation factor, Colony-stimulating factor eosinophil, BCGF-I

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤1.5 ng/mL.

14.2 kDa (124aa)

IL5, ATGP3510-10 µg, ATGP3510-20 µg, ATGP3510-50 µg, ATGP3510-100 µg, ATGP3510-250 µg, ATGP3510-500 µg, ATGP3510-1 mg, ATGP3510-10, ATGP3510-20, ATGP3510-50, ATGP3510-100, ATGP3510-250, ATGP3510-500, ATGP3510-1

Martinez-Moczygemba M., et al. (2003) J Allergy Clin Immunol. 112:653-665.; ; Milburn MV., et al. (1993) Nature. 363:172-176.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_000870

P05113

Human

Ala

IPTEIPT SALVKETLAL LSTHRTLLIA NETLRIPVPV HKNHQLCTEE IFQGIGTLES QTVQGGTVER LFKNLSLIKK YIDGQKKKCG EERRRVNQFL DYLQEFLGVM NTEWIIES