Recombinant humaan IL-9 eiwit

IL-9, also known as interleukin 9, is a cytokine (cell signalling molecule) belonging to the group ofinterleukins. This protein produced by T-cells and specifically by CD4+ helper cells that acts as a regulatorof a variety of hematopoietic cells and stimulates cell proliferation and prevents apoptosis. It functions throughthe interleukin-9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. Recombinant human IL-9, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human IL-9 stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range ≤0.3 ng/mL.

IL-9, Interleukin 9, Cytokine P40, T-cell growth factor P40, p40 T-cell and mast cell growth factor, Homolog of mouse T cell and mast cell growth factor 40, HP40, P40

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by BCA assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ≤0.3 ng/mL.

14.9 kDa (132aa)

IL9, ATGP3912-10 µg, ATGP3912-20 µg, ATGP3912-50 µg, ATGP3912-100 µg, ATGP3912-250 µg, ATGP3912-500 µg, ATGP3912-1 mg, ATGP3912-10, ATGP3912-20, ATGP3912-50, ATGP3912-100, ATGP3912-250, ATGP3912-500, ATGP3912-1

Purwar R, Schlapbach C, et al. (2012) . Nat Med. 18 (8) :1248-53. ; ; Pilette C, Ouadrhiri Y, et al. (2002) . J Immunol. 168 (8) :4103-11.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Hormones & Cytokines

NP_000581

P15248

Human

Gln19