Recombinant humaan Immunoglobuline J-keten/IGJ-eiwit

Immunoglobulin J chain, also known as IGJ, serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also helps to bind these immunoglobulins to secretory component. Recombinant human IGJ protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Joining chain of multimeric IgA and IgM, Immunoglobulin J chain, IgJ chain, J Chain, IGCJ, JCH, IGJ, JCHAIN

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

18 kDa (160aa) confirmed by MALDI-TOF

IGJ, Immunoglobulin J chain, IGCJ, JCH, ATGP2039-10 µg, ATGP2039-20 µg, ATGP2039-50 µg, ATGP2039-100 µg, ATGP2039-250 µg, ATGP2039-500 µg, ATGP2039-1 mg, ATGP2039-10, ATGP2039-20, ATGP2039-50, ATGP2039-100, ATGP2039-250, ATGP2039-500, ATGP2039-1

Liu T., et al. (2005) J. Proteome Res. 4:2070-2080; ; Ramachandran P., et al. (2006) J. Proteome Res. 5:1493-1503;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Immunology

NP_653247.1

P01591

Human

QEDERIV LVDNKCKCAR ITSRIIRSSE DPNEDIVERN IRIIVPLNNR ENISDPTSPL RTRFVYHLSD LCKKCDPTEV ELDNQIVTAT QSNICDEDSA TETCYTYDRN KCYTAVVPLV YGGETKMVET ALTPDACYPD