Recombinant humaan IMPA1/IMP1-eiwit

IMPA1 (inositol monophosphatase1) is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. It plays a key role in the phosphatidylinositol signaling pathway by catalyzing the hydrolysis of inositol monophosphates. This protein has been identified as the pharmacological target for lithium action in the brain. Recombinant human IMPA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Inositol monophosphatase 1, IMPA, IMP, IMPase 1, Inositol monophosphatase 1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

32.3 kDa (297aa) confirmed by MALDI-TOF

IMPA1, IMP1, ATGP0737-10 µg, ATGP0737-20 µg, ATGP0737-50 µg, ATGP0737-100 µg, ATGP0737-250 µg, ATGP0737-500 µg, ATGP0737-1 mg, ATGP0737-10, ATGP0737-20, ATGP0737-50, ATGP0737-100, ATGP0737-250, ATGP0737-500, ATGP0737-1

Schapiro MB., et al. (2002) Neurobiol Aging. 23 (3) :389-96.; ; Atack JR., et al. (1992) Proc Natl Acad Sci u S A. 89 (21) :10031-5.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Metabolism

NP_005527

P29218

Human

MADPWQECMD YAVTLARQAG EVVCEAIKNE MNVMLKSSPV DLVTATDQKV EKMLISSIKE KYPSHSFIGE ESVAAGEKSI LTDNPTWIID PIDGTTNFVH RFPFVAVSIG FAVNKKIEFG VVYSCVEGKM YTARKGKGAF CNGQKLQVSQ QEDITKSLLV TELGSSRTPETVRMVLSNME KLFCIPVHGI RSVGTAAVNM CLVATGGADA YYEMGIHCWD VAGAGIIVTE AGGVLMDVTG GPFDLMSRRV IAANNRILAE RIAKEIQVIP LQRDDED