Recombinant humaan IMPACT-eiwit

IMPACT belongs to the IMPACT family and contains 1 RWD domain. This protein is translational regulator that ensures constant high levels of translation under amino acid starvation and acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis. Recombinant human IMPACT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein IMPACT, RWDD5

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

38.9 kDa (343aa) confirmed by MALDI-TOF

IMPACT, protein IMPACT, RWDD5, ATGP2266-10 µg, ATGP2266-20 µg, ATGP2266-50 µg, ATGP2266-100 µg, ATGP2266-250 µg, ATGP2266-500 µg, ATGP2266-1 mg, ATGP2266-10, ATGP2266-20, ATGP2266-50, ATGP2266-100, ATGP2266-250, ATGP2266-500, ATGP2266-1

Habibi, D., et al. (2010) J. Cell. Physiol. 225 (1), 196-205; ; Pereira, C.M., et al. (2005) J. Biol. Chem. 280 (31), 28316-28323

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_060909

Q9P2X3

Human

MGSSHHHHHH SSGLVPRGSH MGSMAEGDAG SDQRQNEEIE AMAAIYGEEW CVIDDCAKIF CIRISDDIDD PKWTLCLQVM LPNEYPGTAP PIYQLNAPWL KGQERADLSN SLEEIYIQNI GESILYLWVE KIRDVLIQKS QMTEPGPDVK KKTEEEDVEC EDDLILACQP ESSVKALDFD ISETRTEVEV EELPPIDHGI PITDRRSTFQ AHLAPVVCPK QVKMVLSKLY ENKKIASATH NIYAYRIYCE DKQTFLQDCE DDGETAAGGR LLHLMEILNV KNVMVVVSRW YGGILLGPDR FKHINNCARN ILVEKNYTNS PEESSKALGK NKKVRKDKKR NEH