Recombinant humaan IMPAD1-eiwit

IMPAD1, also known as Inositol monophosphatase 3, is a member of the inositol monophosphatase family. IMPAD1 is localized to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP) . Mutations in this gene are a cause of GRAPP type chondrodysplasia with joint dislocations, and a pseudogene of this gene is located on the long arm of chromosome 1. Recombinant human IMPAD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Specific acitivty > 3300 pmol/min/ug, its ability to dephosphorylate adenosine 3'5'-diphosphate sodium slat at pH 7.5, 25C.

Inositol monophosphatase 3, GPAPP, IMP 3, IMP-3, IMPA3

E.coli

Human

His-Tag

Enzyme Activity, SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific acitivty > 3300pmol/min/ug, its ability to dephosphorylate adenosine 3'5'-diphosphate sodium slat at pH 7.5, 25C.

37.6 kDa (349aa) confirmed by MALDI-TOF

IMPAD1, Inositol monophosphatase 3, GPAPP, IMP 3, IMP-3, IMPA3, ATGP2986-10 µg, ATGP2986-20 µg, ATGP2986-50 µg, ATGP2986-100 µg, ATGP2986-250 µg, ATGP2986-500 µg, ATGP2986-1 mg, ATGP2986-10, ATGP2986-20, ATGP2986-50, ATGP2986-100, ATGP2986-250, ATGP2986-500, ATGP2986-1

Vissers L E., et al. (2011) Am J Hum Genet. 88:608-615. ; ; Kalujnaia S., et al. (2010) FASEB J. 24:3981-3991.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Cardiovascular

NP_060283

Q9NX62

Human

MGSSHHHHHH SSGLVPRGSH MGSGRFSLFG LGGEPGGGAA GPAAAADGGT VDLREMLAVS VLAAVRGGDE VRRVRESNVL HEKSKGKTRE GAEDKMTSGD VLSNRKMFYL LKTAFPSVQI NTEEHVDAAD QEVILWDHKI PEDILKEVTT PKEVPAESVT VWIDPLDATQ EYTEDLRKYV TTMVCVAVNG KPMLGVIHKP FSEYTAWAMV DGGSNVKARS SYNEKTPRIV VSRSHSGMVK QVALQTFGNQ TTIIPAGGAG YKVLALLDVP DKSQEKADLY IHVTYIKKWD ICAGNAILKA LGGHMTTLSG EEISYTGSDG IEGGLLASIR MNHQALVRKL PDLEKTGHK