Recombinant humaan IPP-3/PPP1R11-eiwit

Protein phosphatase 1 regulatory subunit 11, also known as PPP1R11, is a 126 amino acid protein that is expressed in a variety of both adult and fetal tissues. PPP1R11 functions as an inhibitor of PP1 (protein phosphatase 1), specifically exhibiting a sensitivity toward the metal-independent and metal-dependent forms of PP1. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Recombinant human PPP1R11 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein phosphatase 1 regulatory inhibitor subunit 11, E3 ubiquitin-protein ligase PPP1R11, Hemochromatosis candidate gene V protein, HCG V, Protein phosphatase 1 regulatory subunit 11, Protein phosphatase inhibitor 3, TCTE5, Tctex5, CFAP255

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

16.3 kDa (149aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

PPP1R11, Protein phosphatase 1 regulatory subunit 11, HCG-V, HCGV, IPP3, TCTE5, TCTEX5, ATGP2173-10 µg, ATGP2173-20 µg, ATGP2173-50 µg, ATGP2173-100 µg, ATGP2173-250 µg, ATGP2173-500 µg, ATGP2173-1 mg, ATGP2173-10, ATGP2173-20, ATGP2173-50, ATGP2173-100, ATGP2173-250, ATGP2173-500, ATGP2173-1

Giffon T., et al. (1996) Immunogenrtics. 44:331-339; ; Zhang J., et al. (1998) Biochemistry. 37:16728-16734.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT

Signal Transduction

NP_068778

O60927

Human

MGSSHHHHHH SSGLVPRGSH MGSMAEAGAG LSETVTETTV TVTTEPENRS LTIKLRKRKP EKKVEWTSDT VDNEHMGRRS SKCCCIYEKP RAFGESSTES DEEEEEGCGH THCVRGHRKG RRRATLGPTP TTPPQPPDPS QPPPGPMQH