Recombinant humaan ISCU-eiwit

Iron-sulfur cluster assembly enzyme, also known as ISCu, is a member of the nifu family. Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. It is interact with ISCS, a cysteine desulfurase, to sequester inorganic sulfur for Fe-S cluster assembly. ISCu-ISCS protein complex localizes in both mitochondria and cytosol, implying that Fe-S cluster assembly takes place in multiple subcellular compartments in mammalian cells. Recombinant human ISCu protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Iron-sulfur cluster assembly enzyme, NIFUN, NifU-like N-terminal domain containing protein, NifU-like protein, IscU iron-sulfur cluster scaffold homolog, iron-sulfur cluster scaffold homolog, ISU2, hnifU

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

16.7 kDa (154aa) confirmed by MALDI-TOF

ISCu, Iron-sulfur cluster assembly enzyme ISCu, HML, hnifu, ISu2, NIFu, NIFuN, ATGP0842-10 µg, ATGP0842-20 µg, ATGP0842-50 µg, ATGP0842-100 µg, ATGP0842-250 µg, ATGP0842-500 µg, ATGP0842-1 mg, ATGP0842-10, ATGP0842-20, ATGP0842-50, ATGP0842-100, ATGP0842-250, ATGP0842-500, ATGP0842-1

Tong WH., et al. (2000) EMBO J. 19:5692-5700; ; Tong WH, et al. (2006) . Cell Metab. 3 (3) : 199-210.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl

Enzymes & Proteases

NP_998760

Q9H1K1

Human

YHKKVVDHY ENPRNVGSLD KTSKNVGTGL VGAPACGDVM KLQIQVDEKG KIVDARFKTF GCGSAIASSS LATEWVKGKT VEEALTIKNT DIAKELCLPP VKLHCSMLAE DAIKAALADY KLKQEPKKGE AEKK