Recombinant humaan isocitraat dehydrogenase 1/IDH1-eiwit

IDH1, also known as isocitrate dehydrogenase (IDHC) cytoplasmic enzyme, belongs to the isocitrate and isopropylmalate dehydrogenases family. This protein catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming alpha-ketoglutarate and CO2 in a two step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of alpha-ketoglutarate. Recombinant human IDH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 18,000 pmol/min/ug. One unit will oxidize 1.0 pmole of DL-Isocitrate to D alpha-Ketoglutarate per minute in the presence of beta-NADP at pH7.4 at 37C.

Isocitrate dehydrogenase [NADP] cytoplasmic, IDCD, IDH, IDP, IDPC, PICD

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 18,000pmol/min/ug. One unit will oxidize 1.0pmole of DL-Isocitrate to D alpha-Ketoglutarate per minute in the presence of beta-NADP at pH7.4 at 37C.

48.8 kDa (434aa) confirmed by MALDI-TOF

Isocitrate Dehydrogenase1, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDCD, IDH, IDP, IDPC, PICD, ATGP1284-10 µg, ATGP1284-20 µg, ATGP1284-50 µg, ATGP1284-100 µg, ATGP1284-250 µg, ATGP1284-500 µg, ATGP1284-1 mg, ATGP1284-10, ATGP1284-20, ATGP1284-50, ATGP1284-100, ATGP1284-250, ATGP1284-500, ATGP1284-1

Thorsness P.E. et al. (1987) J. Biol. Chem. 262: 10422-10425.; ; Geisbrecht B.V. et al. (1999) J. Biol. Chem. 274: 30527-30533.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT,0.1mM PMSF

Cancer

NP_005887.2

O75874

Human

MSKKISGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD ATNDQVTKDA AEAIKKHNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL VSGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPSD GTQKVTYLVH NFEEGGGVAM GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSQFE AQKIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWTRGL AHRAKLDNNK ELAFFANALE EVSIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLKIKLA QAKL