Recombinant humaan ITPA-eiwit

ITPA, also known as Inosine triphosphate pyrophosphatase, is an enzyme that catalyzes the pyrophosphohydrolysis of both ITP (inosine triphosphate) and dITP (deoxyinosine triphosphate) to IMP (inosine monophosphate) and diphosphate. IMP can be used as a substrate for purine nucleotide pathways. IMP can be phosphorylated to ITP, and ITPA can regulate the concentration of ITP in the cell by converting ITP back to IMP. Defects in ITPA result in ITPA deficiency which is thought to be inherited and is characterized by an over-accumulation of ITP in erythocytes, leukocytes and fibroblasts. Recombinant human ITPA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Inosine triphosphate pyrophosphatase, C20orf37, dJ794I6.3, HLC14-06-P, ITPase, My049, OK/SW-cl.9, Inosine triphosphate pyrophosphatase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

23.7 kDa (215aa) confirmed by MALDI-TOF

ITPA, Inosine triphosphate pyrophosphatase, C20orf37, dJ794I6.3, HLC14-06-P, ITPase, My049, OK/SW-cl.9, Inosine triphosphate pyrophosphatase, ATGP0639-10 µg, ATGP0639-20 µg, ATGP0639-50 µg, ATGP0639-100 µg, ATGP0639-250 µg, ATGP0639-500 µg, ATGP0639-1 mg, ATGP0639-10, ATGP0639-20, ATGP0639-50, ATGP0639-100, ATGP0639-250, ATGP0639-500, ATGP0639-1

Clawson GA.. et al. (1991) Cell Growth Differ. 2 (11) :575-82.; ; Lin S., et al. (2001) J Biol Chem.. 276 (22) :18695-701.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Enzymes & Proteases

NP_258412

Q9BY32

Human

MGSSHHHHHH SSGLVPRGSH MMAASLVGKK IVFVTGNAKK LEEVVQILGD KFPCTLVAQK IDLPEYQGEP DEISIQKCQE AVRQVQGPVL VEDTCLCFNA LGGLPGPYIK WFLEKLKPEG LHQLLAGFED KSAYALCTFA LSTGDPSQPV RLFRGRTSGR IVAPRGCQDF GWDPCFQPDG YEQTYAEMPK AEKNAVSHRF RALLELQEYF GSLAA