Recombinant humaan ITPK1-eiwit

Inositol-tetrakisphosphate 1-kinase, also known as ITPK1, is a 414 amino acid monomer that belongs to the ITPK1 family and exists as two alternatively spliced isoforms. ITPK1 is found at highest levels in brain, followed by heart, skeletal muscle, kidney, pancreas, liver, placenta and lung. ITPK1 contains one ATP-grasp domain and has been found to phosphorylate various inositol polyphosphates and modify TNFalpha induced apoptosis. Recombinant human ITPK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Inositol-tetrakisphosphate 1-kinase, ITRPK1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

48.1 kDa (438aa) confirmed by MALDI-TOF

ITPK1, Inositol-tetrakisphosphate 1-kinase, ITRPK1, ATGP1655-10 µg, ATGP1655-20 µg, ATGP1655-50 µg, ATGP1655-100 µg, ATGP1655-250 µg, ATGP1655-500 µg, ATGP1655-1 mg, ATGP1655-10, ATGP1655-20, ATGP1655-50, ATGP1655-100, ATGP1655-250, ATGP1655-500, ATGP1655-1

Sun Y., et al. (2002) J Biol Chem. 277: 45759-45764; ; Chamberlain P P., et al. (2007) J Biol Chem. 282: 28117-28125.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM EDTA

Signal Transduction

NP_055031.2

Q13572

Human

MQTFLK GKRVGYWLSE KKIKKLNFQA FAELCRKRGM EVVQLNLSRP IEEQGPLDVI IHKLTDVILE ADQNDSQSLE LVHRFQEYID AHPETIVLDP LPAIRTLLDR SKSYELIRKI EAYMEDDRIC SPPFMELTSL CGDDTMRLLE KNGLTFPFIC KTRVAHGTNS HEMAIVFNQE GLNAIQPPCV VQNFINHNAV LYKVFVVGES YTVVQRPSLK NFSAGTSDRE SIFFNSHNVS KPESSSVLTE LDKIEGVFER PSDEVIRELS RALRQALGVS LFGIDIIINN QTGQHAVIDI NAFPGYEGVS EFFTDLLNHI ATVLQGQSTA MAATGDVALL RHSKLLAEPA GGLVGERTCS ASPGCCGSMM GQDAPWKAEA DAGGTAKLPH QRLGCNAGVS PSFQQHCVAS LATKASSQ