Recombinant humaan IVD-eiwit

IVD (Isovaleryl Coenzyme A dehydrogenase) is a mitochondrial matrix enzyme that belongs to the acyl-CoA dehydrogenase family. IVD is a homotetrameric flavoenzyme which catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA. Defects of the IVD gene lead to ineffective isoforms that are the underlying cause of isovaleric acidemia. Recombinant human IVD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Isovaleryl-CoA dehydrogenase mitochondrial, ACAD2, Isovaleryl-CoA dehydrogenase, mitochondrial Isovaleryl CoA dehydrogenase, Isovaleryl CoA dehydrogenase, mitochondrial, isovaleryl Coenzyme A dehydrogenase.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

45.3 kDa (415aa) confirmed by MALDI-TOF

IVD, ATGP0487-10 µg, ATGP0487-20 µg, ATGP0487-50 µg, ATGP0487-100 µg, ATGP0487-250 µg, ATGP0487-500 µg, ATGP0487-1 mg, ATGP0487-10, ATGP0487-20, ATGP0487-50, ATGP0487-100, ATGP0487-250, ATGP0487-500, ATGP0487-1

Hodges PW., et al. (2009) Eur J Neurosci. 29 (7) :1490-500. ; ; Rohini R., et al. (2009) Eur J Med Chem. 44 (8) :3330-9.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Redox Proteins

NP_002216.2

P26440

Human

MHSLLPVDDA INGLSEEQRQ LRQTMAKFLQ EHLAPKAQEI DRSNEFKNLR EFWKQLGNLG VLGITAPVQY GGSGLGYLEH VLVMEEISRA SGAVGLSYGA HSNLCINQLV RNGNEAQKEK YLPKLISGEY IGALAMSEPN AGSDVVSMKL KAEKKGNHYI LNGNKFWITN GPDADVLIVY AKTDLAAVPA SRGITAFIVE KGMPGFSTSK KLDKLGMRGS NTCELIFEDC KIPAANILGH ENKGVYVLMS GLDLERLVLA GGPLGLMQAV LDHTIPYLHV REAFGQKIGH FQLMQGKMAD MYTRLMACRQ YVYNVAKACD EGHCTAKDCA GVILYSAECA TQVALDGIQC FGGNGYINDF PMGRFLRDAK LYEIGAGTSE VRRLVIGRAF NADFH