Recombinant humaan IYD-eiwit

IYD is an enzyme that catalyzes the oxidative NADPH-dependent deiodination of mono- and diiodotyrosine, which are the halogenated byproducts of thyroid hormone production. The N-terminus of the protein functions as a membrane anchor. Mutations in this gene cause congenital hypothyroidism due to dyshormonogenesis type 4, which is also referred to as deiodinase deficiency, or iodotyrosine dehalogenase deficiency, or thyroid hormonogenesis type 4. Alternative splicing results in multiple transcript variants. Recombinant human IYD protein, fused to His-tag at N-terminus, was expressed in E. coli.

Iodotyrosine dehalogenase 1 isoform 3, C6orf71, DEHAL1, dJ422F24.1, TDH4

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.1 kDa (214aa)

IYD, iodotyrosine dehalogenase 1 isoform 3, C6orf71, DEHAL1, dJ422F24.1, TDH4, ATGP2592-10 µg, ATGP2592-20 µg, ATGP2592-50 µg, ATGP2592-100 µg, ATGP2592-250 µg, ATGP2592-500 µg, ATGP2592-1 mg, ATGP2592-10, ATGP2592-20, ATGP2592-50, ATGP2592-100, ATGP2592-250, ATGP2592-500, ATGP2592-1

Burniat, A, et al. (2012) J. Clin. Endocrinol. Metab. 97 (7), E1276-E1283; ; Belevich, V.K., et al. (2011) Biomed Khim 57 (2), 187-194;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Cardiovascular

NP_001158167

Q6PHW0

Human

DRSMEKK KGEPRTRAEA RPWVDEDLKD SSDLHQAEED ADEWQESEEN VEHIPFSHNH YPEKEMVKRS QEFYELLNKR RSVRFISNEQ VPMEVIDNVI RTAGTAPSGA HTEPWTFVVV KDPDVKHKIR KIIEEEEEIN YMKRMGHRWV TDLKKLRTNW IKEYLDTAPI LILIFKQVHG FAANGKKKVH YYNE