Recombinant humaan JAM-A/F11R-eiwit

F11R, also known as CD321, belongs to the immunoglobulin superfamily. It seems to plays a role in epithelial tight junction formation. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. F11R protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Recombinant human F11R protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Junctional adhesion molecule A, JAM-A, Junctional adhesion molecule 1, JAM-1, Platelet F11 receptor, Platelet adhesion molecule 1, PAM-1, CD321, F11R, JAM1, JCAM

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 90% by SDS-PAGE

25.8 kDa (238aa) confirmed by MALDI-TOF

JAM-A, Junctional adhesion molecule A, CD321, JAM, JAM1, JAMA, JCAM, KAT, PAM-1, ATGP1556-10 µg, ATGP1556-20 µg, ATGP1556-50 µg, ATGP1556-100 µg, ATGP1556-250 µg, ATGP1556-500 µg, ATGP1556-1 mg, ATGP1556-10, ATGP1556-20, ATGP1556-50, ATGP1556-100, ATGP1556-250, ATGP1556-500, ATGP1556-1

Murakami, M., et al. (2011) PLoS ONE 6 (6), E21242

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.15M NaCl,1mM DTT

Cytoskeleton

NP_058642

Q9Y624

Human

LGSVT VHSSEPEVRI PENNPVKLSC AYSGFSSPRV EWKFDQGDTT RLVCYNNKIT ASYEDRVTFL PTGITFKSVT REDTGTYTCM VSEEGGNSYG EVKVKLIVLV PPSKPTVNIP SSATIGNRAV LTCSEQDGSP PSEYTWFKDG IVMPTNPKST RAFSNSSYVL NPTTGELVFD PLSASDTGEY SCEARNGYGT PMTSNAVRME AVERNVGV