Recombinant humaan JAM-B/VE-JAM-eiwit

JAM2, also known as junctional adhesion molecule B isoform 1, is a type 1 transmembrane receptor belonging to the immunoglobulin superfamily. This protein is expressed prominently on high endothelial venules of lymphoid organs where it is localized to the intercellular boundaries of high endothelial cells. It can function as an adhesive ligand for the T cell line J45 and can interact with GM-CSF/IL-4-delived peripheral blood dendritic cells. Also, it plays a role in the regulation of transendothelial migration. It binds to very late activation antigen (VLA) -4, a leucocyte integrin that contributes to rolling and firm adhesion of lymphocytes to endothelial cells through binding to vascular cell adhesion molecule (VCAM) -1. Recombinant human JAM2, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Junctional adhesion molecule B isoform 1, JAM2, C21orf43, CD322, JAM-B, JAMB, PRO245, VE-JAM, VEJAM

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

50.7 kDa (452aa)

JAM-B, VE-JAM, Junctional adhesion molecule B isoform 1, JAM2, C21orf43, CD322, JAM-B, JAMB, PRO245, VE-JAM, VEJAM, ATGP3758-10 µg, ATGP3758-20 µg, ATGP3758-50 µg, ATGP3758-100 µg, ATGP3758-250 µg, ATGP3758-500 µg, ATGP3758-1 mg, ATGP3758-10, ATGP3758-20, ATGP3758-50, ATGP3758-100, ATGP3758-250, ATGP3758-500, ATGP3758-1

Palmeri D., et al, (2000) J. Biol. Chem. 275:19139-19145.; ; Cunningham SA., et al, (2000) J. Biol. Chem. 275:34750-34756.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 50% glycerol, 1mM DTT

Signal Transduction

NP_067042

P57087

Human

Ala

ADPFSAPKDQ QVVTAVEYQE AILACKTPKK TVSSRLEWKK LGRSVSFVYY QQTLQGDFKN RAEMIDFNIR IKNVTRSDAG KYRCEVSAPS EQGQNLEEDT VTLEVLVAPA VPSCEVPSSA LSGTVVELRC QDKEGNPAPE YTWFKDGIRL LENPRLGSQS TNSSYTMNTK TGTLQFNTVS KLDTGEYSCE ARNSVGYRRC PGKRMQVDDL NISLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH