Recombinant humaan JAM-C eiwit

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. JAM3 is localized in the tight junctions between high endothelial cells. unlike other proteins in this family, this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. JAM3 is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. Recombinant human JAM3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Junctional adhesion molecule C, Junctional adhesion molecule 3, JAM-C, JAMC

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26 kDa (234aa) confirmed by MALDI-TOF

JAM-C, Junctional adhesion molecule C, Junctional adhesion molecule 3, JAM-C, JAMC, ATGP1723-10 µg, ATGP1723-20 µg, ATGP1723-50 µg, ATGP1723-100 µg, ATGP1723-250 µg, ATGP1723-500 µg, ATGP1723-1 mg, ATGP1723-10, ATGP1723-20, ATGP1723-50, ATGP1723-100, ATGP1723-250, ATGP1723-500, ATGP1723-1

Santoso S., et al. (2002) . J Exp Med. 2; ;196 (5) :679-91.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM EDTA, 5mM DTT

Signal Transduction

NP_116190

Q9BX67

Human

VNLKSS NRTPVVQEFE SVELSCIITD SQTSDPRIEW KKIQDEQTTY VFFDNKIQGD LAGRAEILGK TSLKIWNVTR RDSALYRCEV VARNDRKEID EIVIELTVQV KPVTPVCRVP KAVPVGKMAT LHCQESEGHP RPHYSWYRND VPLPTDSRAN PRFRNSSFHL NSETGTLVFT AVHKDDSGQY YCIASNDAGS ARCEEQEMEV YDLN