Recombinant humaan JNK1-eiwit

MAPK8 also known as mitogen-activated protein kinase 8 JNK beta 2. MAPK8 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. Recombinant human MAPK8, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Mitogen-activated protein kinase 8 JNK beta 2, JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

> 85% by SDS-PAGE

50.5 kDa (450aa)

JNK1, Mitogen-activated protein kinase 8 JNK beta 2, JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c, ATGP3120-10 µg, ATGP3120-20 µg, ATGP3120-50 µg, ATGP3120-100 µg, ATGP3120-250 µg, ATGP3120-500 µg, ATGP3120-1 mg, ATGP3120-10, ATGP3120-20, ATGP3120-50, ATGP3120-100, ATGP3120-250, ATGP3120-500, ATGP3120-1

Jha A., et al. (2014) EMBO J. 33 (5), 501-511; ; Misheva M., et al. (2014) Biochim. Biophys. Acta 1843 (2), 253-264;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Signal Transduction

NP_001265476

P45983

Human

MGSSHHHHHH SSGLVPRGSH MGSMSRSKRD NNFYSVEIGD STFTVLKRYQ NLKPIGSGAQ GIVCAAYDAI LERNVAIKKL SRPFQNQTHA KRAYRELVLM KCVNHKNIIG LLNVFTPQKS LEEFQDVYIV MELMDANLCQ VIQMELDHER MSYLLYQMLC GIKHLHSAGI IHRDLKPSNI VVKSDCTLKI LDFGLARTAG TSFMMTPYVV TRYYRAPEVI LGMGYKENVD IWSVGCIMGE MIKGGVLFPG TDHIDQWNKV IEQLGTPCPE FMKKLQPTVR TYVENRPKYA GYSFEKLFPD VLFPADSEHN KLKASQARDL LSKMLVIDAS KRISVDEALQ HPYINVWYDP SEAEAPPPKI PDKQLDEREH TIEEWKELIY KEVMDLEERT KNGVIRGQPS PLGAAVINGS QHPSSSSSVN DVSSMSTDPT LASDTDSSLE AAAGPLGCCR