Recombinant humaan JNK3/MAPK10-eiwit

MAPK10, also known as mitogen-activated protein kinase 10, is a member of the MAP kinase family. This group of protein kinases includes at least 10 members that interact selectively with ATF2, Jun and Elk-i transcription factors. It acts as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs) . Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Also, expression of multiple JNK isoforms provides a mechanism for the generation of tissue-specific responses to the activation of the JNK signal transduction pathway. Recombinant human MAPK10, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Mitogen-activated protein kinase 10, MAPK10, JNK3, JNK3A, p493F12, p54bSAPK, PRKM10, SAPK1b

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

53.4 kDa (470aa)

MAPK10, Mitogen-activated protein kinase 10, MAPK10, JNK3, JNK3A, p493F12, p54bSAPK, PRKM10, SAPK1b, ATGP3837-10 µg, ATGP3837-20 µg, ATGP3837-50 µg, ATGP3837-100 µg, ATGP3837-250 µg, ATGP3837-500 µg, ATGP3837-1 mg, ATGP3837-10, ATGP3837-20, ATGP3837-50, ATGP3837-100, ATGP3837-250, ATGP3837-500, ATGP3837-1

Mohit AA., et al, (1995) Neuron 14:67-78.; ; Gupta S., et al, (1996) EMBO J. 15:2760-2770.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT, 0.1mM PMSF

Neuroscience

NP_620448

P53779

Human

Met