Recombinant humaan Josephine 1/JOSD1-eiwit

JOSD1, also as known as Josephin-1, has low protease activity towards poly-ubiquitin chains (in vitro) . JOSD1 is act as a deubiquitinating enzyme. Recently identified ubiquitin-binding sites in the Josephin domain contribute to ubiquitin chain binding and cleavage. Recombinant human JOSD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Josephin-1, dJ508I15.2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.6 kDa (225aa) confirmed by MALDI-TOF

Josephin1, Josephin-1, dJ508I15.2, ATGP1756-10 µg, ATGP1756-20 µg, ATGP1756-50 µg, ATGP1756-100 µg, ATGP1756-250 µg, ATGP1756-500 µg, ATGP1756-1 mg, ATGP1756-10, ATGP1756-20, ATGP1756-50, ATGP1756-100, ATGP1756-250, ATGP1756-500, ATGP1756-1

Weeks S.D., et al. (2011) J. Biol. Chem. 286:4555-4565

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 5mM DTT, 2mM EDTA

Ubiquitination

NP_055691

Q15040

Human

MSCVPWK GDKAKSESLE LPQAAPPQIY HEKQRRELCA LHALNNVFQD SNAFTRDTLQ EIFQRLSPNT MVTPHKKSML GNGNYDVNVI MAALQTKGYE AVWWDKRRDV GVIALTNVMG FIMNLPSSLC WGPLKLPLKR QHWICVREVG GAYYNLDSKL KMPEWIGGES ELRKFLKHHL RGKNCELLLV VPEEVEAHQS WRTDV