Recombinant humaan JTB-eiwit

Jumping translocation breakpoint, also known as JTB, is required for normal cytokinesis during mitosis. This protein plays a role in the regulation of cell proliferation. This protein may be a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. It lnhibits apoptosis induced by TGFB1. Overexpression induces swelling of mitochondria and reduces mitochondrial membrane potential. Recombinant human JTB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Jumping translocation breakpoint, hJT, HJTB, HSPC222, PAR

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

10.7 kDa (98aa) confirmed by MALDI-TOF

JTB, Jumping translocation breakpoint, hJT, HJTB, HSPC222, PAR, ATGP2782-10 µg, ATGP2782-20 µg, ATGP2782-50 µg, ATGP2782-100 µg, ATGP2782-250 µg, ATGP2782-500 µg, ATGP2782-1 mg, ATGP2782-10, ATGP2782-20, ATGP2782-50, ATGP2782-100, ATGP2782-250, ATGP2782-500, ATGP2782-1

Platica M., et al. (2011) Int. J. Oncol. 38:777-785; ; Kanome T., et al. (2007) Oncogene. 26:5991-6001

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Other

NP_006685

O76095

Human

EAPVQEE KLSASTSNLP CWLVEEFVVA EECSPCSNFR AKTTPECGPT GYVEKITCSS SKRNEFKSCR SALMEQRL