Recombinant humaan K-Ras-eiwit

KRAS is a member of the small GTPase superfamily. This protein is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. under normal conditions, Ras family members influence cell growth and differentiation events in a subcellular membrane compartmentalization-based signaling system. Oncogenic Ras can deregulate processes that control both cell proliferation and apoptosis. Recombinant human KRAS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

GTPase Kras, KRAS proto-oncogene GTPase, KRAS2, v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, Kirsten rat sarcoma viral oncogene homolog, KRAS1, K-Ras4B, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, RASK2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.8 kDa (210aa) confirmed by MALDI-TOF

KRAS, GTPase KRas isoform a precursor, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2, ATGP2062-10 µg, ATGP2062-20 µg, ATGP2062-50 µg, ATGP2062-100 µg, ATGP2062-250 µg, ATGP2062-500 µg, ATGP2062-1 mg, ATGP2062-10, ATGP2062-20, ATGP2062-50, ATGP2062-100, ATGP2062-250, ATGP2062-500, ATGP2062-1

Lee W. et al. (2010) Nature. 465:473-477.; ; Singh M. et al. (2010) Nat Biotechnol. 28:585-593.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT

Cancer

NP_203524.1

P01116

Human

MTEYKL VVVGAGGVGK SALTIQLIQN HFVDEYDPTI EDSYRKQVVI DGETCLLDIL DTAGQEEYSA MRDQYMRTGE GFLCVFAINN TKSFEDIHHY REQIKRVKDS EDVPMVLVGN KCDLPSRTVD TKQAQDLARS YGIPFIETSA KTRQRVEDAF YTLVREIRQY RLKKISKEEK TPGCVKIKKC