Recombinant humaan Kallikrein 3/PSA-eiwit

Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a member of the kallikrein-related peptidase family. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This protein is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19 and is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Recombinant human KLK3 protein, fused to His-tag at N-terminus, was expressed in E. coli.

KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, APS, hK3, KLK2A1, Kallikrein related peptidase 3, Prostate-specific antigen isoform 1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

28.8 kDa (262aa)

Seminin, Semenogelase, PSA, Prostate-specific antigen isoform 1, Prostate-specific antigen, Prostate specific antigen isoform 1, Prostate specific antigen, P-30 antigen, P30 antigen, KLK3, KLK2A1, Kallikrein-3, Kallikrein3, Kallikrein related peptidase 3, Kallikrein 3, hK3, Gamma-seminoprotein, APS, Gamma seminoprotein, ATGP1566-10 µg, ATGP1566-20 µg, ATGP1566-50 µg, ATGP1566-100 µg, ATGP1566-250 µg, ATGP1566-500 µg, ATGP1566-1 mg, ATGP1566-010, ATGP1566-020, ATGP1566-050, ATGP1566-100, ATGP1566-250, ATGP1566-500, ATGP1566-01M

Yang Q.F., et al. (2000) J. Hum. Genet. 45:363-366; ; Cramer S.D., et al. (2003) J. Natl. Cancer Inst. 95:1044-1053

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol

Cancer

NP_001639.1

P07288

Human

IVGGW ECEKHSQPWQ VLVASRGRAV CGGVLVHPQW VLTAAHCIRN KSVILLGRHS LFHPEDTGQV FQVSHSFPHP LYDMSLLKNR FLRPGDDSSH DLMLLRLSEP AELTDAVKVM DLPTQEPALG TTCYASGWGS IEPEEFLTPK KLQCVDLHVI SNDVCAQVHP QKVTKFMLCA GRWTGGKSTC SGDSGGPLVC NGVLQGITSW GSEPCALPER PSLYTKVVHY RKWIKDTIVA NP